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IHCPlus™ NEFM / NF‑M Antibody Monoclonal IHC,WB LS‑B7183

Catalog Number Size Price
LS-B7183-0.05 0.05 mg (1 mg/ml) $425 
Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ NEFM / NF‑M Antibody Monoclonal IHC,WB LS‑B7183

Note: This antibody replaces LS-C146777, LS-C150507
Antibody:
Mouse Monoclonal to Human NEFM / NF-M
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Monkey, Hamster, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7183-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human NEFM / NF-M
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Monkey, Hamster, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
NF-M antibody LS-B7183 is an unconjugated mouse monoclonal antibody to NF-M (NEFM ) from human, hamster, monkey and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NEFM / NF-M
Host
Mouse
Reactivity
Human, Monkey, Hamster, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
RNF403
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:50 - 1:100)
  • Western blot (1:100 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Neurofilament preparation of calf brain tissue.
Specificity
Reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFM / NF-M
P07197 NM_005382 NP_005373.2

Validation

Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Featured Products

Anti-NEFM / NF-M antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
Species: Rat, Human, Mouse, Rabbit, Chicken
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Monkey, Guinea pig, Hamster, Rabbit, Xenopus
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Product
Species: Mouse, Human
Applications: IHC
Immunohistochemistry analysis of the neurofilament medium chain showing staining in the filaments of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament medium chain monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Species: Rat, Human, Mouse, Rabbit, Chicken
Applications: IHC, Immunofluorescence, Western blot, ELISA
Reactivity: Human
Range: 15.63-1000 pg/ml

Requested From: United States
Date Requested: 8/19/2019
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