Mouse NCS1 / Neuronal Calcium Sensor

Rabbit

Mouse, Human, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity chromatography

Unmodified

Frequenin (recombinant from Mouse with extensive post-translational modifications)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

• IHC
• IHC - Paraffin (5 µg/ml)
• Immunofluorescence (1:100 - 1:2500)
• Western blot (1:500 - 1:2000)
• ELISA (1:10000 - 1:50000)

Immunohistochemistry: LS-B1457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1457 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P62166 NCBI: NM_014286 NP_055101.2