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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8464-50 50 µg $425 
Muscle Actin Antibody - Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Muscle Actin Antibody - Paraffin section of human cardiac muscle immunostained with HHF35 (1:250)
Muscle Actin Antibody - Immunofluorescence staining of intestinal muscle tissue in 1 month old zebrafish embryo
Muscle Actin Antibody - Immunofluorescence staining of muscle tissue of 7 days old zebrafish embryo. Note reactivity in both striated and smooth muscle tissues Upper panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining
Muscle Actin Antibody - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
Muscle Actin Antibody - Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Muscle Actin Antibody - Paraffin section of human cardiac muscle immunostained with HHF35 (1:250)
Muscle Actin Antibody - Immunofluorescence staining of intestinal muscle tissue in 1 month old zebrafish embryo
Muscle Actin Antibody - Immunofluorescence staining of muscle tissue of 7 days old zebrafish embryo. Note reactivity in both striated and smooth muscle tissues Upper panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining
Muscle Actin Antibody - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
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IHCPlus™ Monoclonal Mouse anti‑Human Muscle Actin Antibody (clone HHF35, IHC, WB) LS‑B8464

IHCPlus™ Monoclonal Mouse anti‑Human Muscle Actin Antibody (clone HHF35, IHC, WB) LS‑B8464

Note: This antibody replaces LS-C146644
Antibody:
Mouse Monoclonal to Human Muscle Actin
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA, EM
Reactivity:
Human, Monkey, Rat, Pig, Rabbit, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8464-50
50 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human Muscle Actin
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA, EM
Reactivity:
Human, Monkey, Rat, Pig, Rabbit, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
Muscle Actin antibody LS-B8464 is an unconjugated mouse monoclonal antibody to Muscle Actin from human. It is reactive with human, rat, chicken and other species. Validated for ELISA, EM, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Muscle Actin
Synonyms
MSA | Muscle Specific Actin | HHF35
Host
Mouse
Reactivity
Human, Monkey, Rat, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
HHF35
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen (1:200 - 1:1000)
  • Western blot (1:1000 - 1:5000)
  • ELISA
  • Electron Microscopy
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SDS-extracted protein fraction from human myocardium
Specificity
Reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections. The epitope recognized by HHF35 is highly conserved. Therefore the antibody cross-reacts with several species including human, monkey, swine, rabbit, rat, chicken and zebrafish
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Muscle Actin Antibody - Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ Muscle Actin Antibody (clone HHF35) for IHC, WB/Western, ELISA LS-B8464 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 5/30/2020