Bovine MSN / Moesin

Mouse

Bovine, Human, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein G purified

Unmodified

Moesin from cow uterus.

Recognizes 78kD moesin. Weakly recognizes 80kD radixin. Detects most of the proteolytic fragments of moesin. Does not react with talin or ezrin.

• IHC
• IHC - Paraffin (5 µg/ml)
• Immunofluorescence
• Western blot (1 - 2 µg/ml)
• Immunoprecipitation
• ELISA

Immunohistochemistry: LS-B2180 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2180 was determined to be 5 ug/ml. Immunoprecipitation: Denatured only. 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml for 2 hours at RT. Positive control: Raji cells, placenta.

PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P26038 NCBI: NM_002444 NP_002435.1