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Catalog Number Size Price
LS-B2688-0.05 0.05 mg (1 mg/ml) $545 
MANF / ARMET Antibody - Anti-MANF / ARMET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
MANF / ARMET Antibody - Immunofluorescence of MANF in Human Brain cells with MANF antibody at 20 ug/ml.
MANF / ARMET Antibody - Western blot of MANF in mouse brain tissue lysate with MANF antibody at (A) 1 and (B) 2 ug/ml.
MANF / ARMET Antibody - Anti-MANF / ARMET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
MANF / ARMET Antibody - Immunofluorescence of MANF in Human Brain cells with MANF antibody at 20 ug/ml.
MANF / ARMET Antibody - Western blot of MANF in mouse brain tissue lysate with MANF antibody at (A) 1 and (B) 2 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human MANF / ARMET Antibody (IHC, IF, WB) LS‑B2688

IHC‑plus™ Polyclonal Rabbit anti‑Human MANF / ARMET Antibody (IHC, IF, WB) LS‑B2688

Note: This antibody replaces LS-C53207
Antibody:
MANF / ARMET Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$545
LS-B2688-0.05
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MANF / ARMET Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ARMET antibody LS-B2688 is an unconjugated rabbit polyclonal antibody to ARMET (MANF) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human MANF / ARMET
Synonyms
MANF | ARP | ARMET | Arginine-rich protein | Protein ARMET
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
A 12 AA synthetic peptide near the C-Terminus of human MANF. The immunogen is located within the last 50 AA of MANF.
Specificity
This antibody does not cross-react with CDNF.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
MANF antibody can be used for detection of MANF by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 20 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MANF / ARMET
P55145 NM_006010 NP_006001.4

Validation

MANF / ARMET Antibody - Anti-MANF / ARMET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MANF / ARMET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MANF / ARMET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ MANF / ARMET Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B2688 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)

Publications (4)

Piperine ameliorates SCA17 neuropathology by reducing ER stress. Guo J, Cui Y, Liu Q, Yang Y, Li Y, Weng L, Tang B, Jin P, Li XJ, Yang S, Li S. Molecular neurodegeneration. 2018 January;13:4. (Human)

Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice. Galli E, Rossi J, Neumann T, Andressoo JO, Drinda S, Lindholm P. Science advances. 2019 October;9:14318.

Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Is Highly Expressed in Mouse Tissues With Metabolic Function. Danilova T, Galli E, Pakarinen E, Palm E, Lindholm P, Saarma M, Lindahl M. Frontiers in endocrinology. 2019 November;10:

MANF Is Neuroprotective in Early Stages of EAE, and Elevated in Spinal White Matter by Treatment With Dexamethasone. Jinhan Nam, Tapani K Koppinen, Merja H Voutilainen. Frontiers in cellular neuroscience. 2021 Jul;15:640084.

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Requested From: United States
Date Requested: 4/16/2024