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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8321-50 50 µg (0.5 mg/ml) $425 
LSP1 Antibody - Anti-LSP1 antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
LSP1 Antibody - Goat Anti-LSP1 Antibody (0.05µg/ml) staining of Human Liver lysate (lane 1), Mouse Liver lysate (lane 2) and Rat Liver lysate (lane 3) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
LSP1 Antibody - LSP1 antibody (0.05 ug/ml) staining of Human Peripheral Lymphocytes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LSP1 Antibody - Anti-LSP1 antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
LSP1 Antibody - Goat Anti-LSP1 Antibody (0.05µg/ml) staining of Human Liver lysate (lane 1), Mouse Liver lysate (lane 2) and Rat Liver lysate (lane 3) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
LSP1 Antibody - LSP1 antibody (0.05 ug/ml) staining of Human Peripheral Lymphocytes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ LSP1 Antibody aa445‑459 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8321

IHCPlus™ LSP1 Antibody aa445‑459 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8321

Note: This antibody replaces LS-C112940
Antibody:
Goat Polyclonal to Human LSP1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8321-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human LSP1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
LSP1 antibody LS-B8321 is an unconjugated goat polyclonal antibody to LSP1 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human LSP1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.05 - 0.2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-RYKFVATGHGKYEKV, from the C Terminus of the protein sequence according to NP_002330.1NP_001013271.1.
Blocking Peptide
LS-E27302 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8321. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa445-459
Specificity
Human LSP1. This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 50kDa band observed in Human Lymph Node, Tonsil and Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med. 2005 Feb 7;201(3):409-18; PMID: 15684321) . Recommended concentration: 0.05-0.2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LSP1
P33241 NM_002339 NP_002330.1

Validation

LSP1 Antibody - Anti-LSP1 antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-LSP1 antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-LSP1 antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Requested From: United States
Date Requested: 11/13/2019