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IHCPlus™ Leptin Antibody N‑Terminus Polyclonal IHC,WB LS‑B4651

Catalog Number Size Price
LS-B4651-100 100 µl $485 
Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LEP / Leptin antibody Western blot of Fetal Small Intestine lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LEP / Leptin antibody Western blot of Fetal Small Intestine lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ Leptin Antibody N‑Terminus Polyclonal IHC,WB LS‑B4651

Note: This antibody replaces LS-C107518
Antibody:
Rabbit Polyclonal to Human Leptin
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B4651-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Leptin
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Leptin antibody LS-B4651 is an unconjugated rabbit polyclonal antibody to Leptin from human, mouse, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Leptin
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from N-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Hamster (100%); Galago, Marmoset, Rat, Opossum (92%); Sheep, Goat (90%); Monkey, Elephant, Zebu, Dog, Cat, Bovine (85%).
Blocking Peptide
LS-E15343 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4651. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human LEP / Leptin
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Leptin
P41159 NM_000230 NP_000221.1

Validation

Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/22/2019