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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IHCPlus™ LEFTYB / LEFTY1 Antibody N‑Terminus Polyclonal IHC,WB LS‑B5830

Catalog Number Size Price
LS-B5830-100 100 µl $485 
Anti-LEFTY1 / Lefty antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LEFTY1 / Lefty antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-LEFTY1 / Lefty antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
LEFTY1 / Lefty antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ LEFTYB / LEFTY1 Antibody N‑Terminus Polyclonal IHC,WB LS‑B5830

Note: This antibody replaces LS-C111390
Antibody:
Rabbit Polyclonal to Human LEFTYB / LEFTY1
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5830-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human LEFTYB / LEFTY1
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
LEFTY1 antibody LS-B5830 is an unconjugated rabbit polyclonal antibody to human LEFTY1 (LEFTYB ). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human LEFTYB / LEFTY1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from N-Terminus of human LEFTY1 (O75610, NP_066277). Percent identity by BLAST analysis: Human (100%); Gorilla (92%); Gibbon, Marmoset (85%).
Blocking Peptide
LS-E18838 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human LEFTY1 / Lefty
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LEFTYB / LEFTY1
O75610 NM_020997 NP_066277.1

Validation

Anti-LEFTY1 / Lefty antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-LEFTY1 / Lefty antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-LEFTY1 / Lefty antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (1)

Quantitative Profiling of Single Formalin Fixed Tumour Sections: proteomics for translational research. Hughes CS, McConechy MK, Cochrane DR, Nazeran T, Karnezis AN, Huntsman DG, Morin GB. Scientific reports. 2016 6:34949. (WB; Human)

Reviews (0)

Featured Products

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with LEFTB antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of Lefty antibody
Species: Human, Mouse
Applications: Western blot, ELISA
Reactivity: Human
Range: 7.8-500 pg/ml
Reactivity: Mouse
Range: 3.125-200 ng/ml
Reactivity: Human
Range: 15.6-1000 pg/ml
Reactivity: Mouse
Range: 6.17-500 pg/ml

Requested From: United States
Date Requested: 8/22/2019
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