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Catalog Number Size Price
LS-B1453-50 50 µg (1 mg/ml) $485 
LDB2 Antibody - Anti-LDB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LDB2 Antibody - Anti-LDB2 Antibody - Western Blot. Western blot of Affinity Purified anti-LDB2 antibody shows detection of a 43-kD band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
LDB2 Antibody - Anti-LDB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LDB2 Antibody - Anti-LDB2 Antibody - Western Blot. Western blot of Affinity Purified anti-LDB2 antibody shows detection of a 43-kD band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
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IHC‑plus™ Polyclonal Rabbit anti‑Mouse LDB2 Antibody (IHC, WB) LS‑B1453

IHC‑plus™ Polyclonal Rabbit anti‑Mouse LDB2 Antibody (IHC, WB) LS‑B1453

Note: This antibody replaces LS-C18946, LS-C71684
Antibody:
LDB2 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human, Chimpanzee, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B1453-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
LDB2 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human, Chimpanzee, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
LDB2 antibody LS-B1453 is an unconjugated rabbit polyclonal antibody to LDB2 from mouse. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Mouse LDB2
Synonyms
LDB2 | CLIM-1 | CLIM1 | LIM binding domain 2 | LIM domain binding 2 | LIM domain-binding protein 2 | LDB-2 | LIM domain-binding factor-2
Host
Rabbit
Reactivity
Mouse, Human, Chimpanzee, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1453 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1453 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LDB2
O43679 NM_001290 NP_001281.1

Validation

LDB2 Antibody - Anti-LDB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LDB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LDB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ LDB2 Antibody for IHC, WB/Western, ELISA LS-B1453 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/27/2021