LDB2 | CLIM-1 | CLIM1 | LIM binding domain 2 | LIM domain binding 2 | LIM domain-binding protein 2 | LDB-2 | LIM domain-binding factor-2
Mouse, Human, Chimpanzee, Rat, Dog, Chicken
(tested or 100% immunogen sequence identity)
Human, Chimpanzee, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.
A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:20000)
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Immunohistochemistry: LS-B1453 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1453 was determined to be 5 ug/ml.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.