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Catalog Number Size Price
LS-B13585-200 200 µl $440 
KLF5 / BTEB2 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
KLF5 / BTEB2 Antibody - Fluorescent confocal image of U251 cell stained with KLF5 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
KLF5 / BTEB2 Antibody - KLF5 Antibody western blot of CEM cell line lysates (35 ug/lane). The KLF5 antibody detected the KLF5 protein (arrow).
KLF5 / BTEB2 Antibody - KLF5 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KLF5 / BTEB2 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
KLF5 / BTEB2 Antibody - Fluorescent confocal image of U251 cell stained with KLF5 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
KLF5 / BTEB2 Antibody - KLF5 Antibody western blot of CEM cell line lysates (35 ug/lane). The KLF5 antibody detected the KLF5 protein (arrow).
KLF5 / BTEB2 Antibody - KLF5 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human KLF5 / BTEB2 Antibody (aa341‑370, IHC, IF, WB) LS‑B13585

IHC‑plus™ Polyclonal Rabbit anti‑Human KLF5 / BTEB2 Antibody (aa341‑370, IHC, IF, WB) LS‑B13585

Note: This antibody replaces LS-C100521
Antibody:
KLF5 / BTEB2 Rabbit anti-Human Polyclonal (aa341-370) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B13585-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
KLF5 / BTEB2 Rabbit anti-Human Polyclonal (aa341-370) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
BTEB2 antibody LS-B13585 is an unconjugated rabbit polyclonal antibody to BTEB2 (KLF5) (aa341-370) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KLF5 / BTEB2
Synonyms
KLF5 | BTE-binding protein 2 | BTEB2 | Colon kruppel-like factor | Colon krueppel-like factor | GC box binding protein 2 | IKLF | Gc box-binding protein bteb2 | GC-box-binding protein 2 | Transcription factor BTEB2 | Krueppel-like factor 5
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa341-370
Specificity
This KLF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human KLF5.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KLF5 / BTEB2
Q13887 NM_001730 NP_001721.2

Validation

KLF5 / BTEB2 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ KLF5 / BTEB2 Antibody (aa341-370) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B13585 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024