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IHCPlus™ KIAA0652 / ATG13 Antibody Polyclonal ELISA,IF,IHC,WB LS‑B5277

Catalog Number Size Price
LS-B5277-0.05 0.05 mg (1 mg/ml) $425 
Anti-ATG13 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of ATG13 in Mouse Heart cells with ATG13 antibody at 20 ug/ml.
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Anti-ATG13 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of ATG13 in Mouse Heart cells with ATG13 antibody at 20 ug/ml.
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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IHCPlus™ KIAA0652 / ATG13 Antibody Polyclonal ELISA,IF,IHC,WB LS‑B5277

Note: This antibody replaces LS-C115850
Antibody:
Rabbit Polyclonal to Human KIAA0652 / ATG13
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5277-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human KIAA0652 / ATG13
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ATG13 antibody LS-B5277 is an unconjugated rabbit polyclonal antibody to ATG13 (KIAA0652 ) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KIAA0652 / ATG13
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
a 15 amino acid synthetic peptide near the C-terminus of human ATG13. The immunogen is located within amino acids 450 - 500 of ATG13.
Blocking Peptide
LS-E30942 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B5277. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
Multiple isoforms of ATG13 are known to exist.
Usage
ATG13 antibody can be used for detection of ATG13 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 44, 53, 57, 61 kDa Observed: 49 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KIAA0652 / ATG13
O75143 NM_014741 NP_001136145.1

Validation

Anti-ATG13 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ATG13 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATG13 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATG13 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Featured Products

Western blot of affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibodyat dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Reactivity: Mouse
Range: 0.313-20 ng/ml
Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
Species: Bovine, Human, Mouse, Rat
Applications: Immunofluorescence, Western blot

Requested From: United States
Date Requested: 9/21/2019