Human ITGB1 / Integrin Beta 1 / CD29

Mouse

Human, Rat (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

HUTS-4

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Purified

Unmodified

Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.

Specific for active conformation of human b1 integrins. Does not augment the affinity of beta 1 for ligands. Species cross-reactivity: rat.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - 4% paraformaldehyde
• Western blot
• Flow Cytometry
• ELISA

Immunohistochemistry: LS-B2861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2861 was determined to be 10 ug/ml. FACS: 2.5 ug/5x10^5 cells.

PBS, pH 7.1. No preservatives.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05556 NCBI: NM_002211 NP_002202.2