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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2732-0.05 | 0.05 mg (1 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human IRGM / LRG‑47 Antibody (IHC, IF, WB) LS‑B2732
IHC‑plus™ Polyclonal Rabbit anti‑Human IRGM / LRG‑47 Antibody (IHC, IF, WB) LS‑B2732
Note: This antibody replaces LS-C53284
Antibody:
IRGM / LRG-47 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IRGM / LRG-47 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
LRG-47 antibody LS-B2732 is an unconjugated rabbit polyclonal antibody to LRG-47 (IRGM) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human IRGM / LRG-47
Synonyms
IRGM | LRG-47-like protein | IFI1 | IRGM1 | LRG47 | LRG-47
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
A 14 AA synthetic peptide near the center of the human IRGM. The immunogen is located within the last 50 AA of IRGM.
Specificity
IRGM antibody may recognize mouse IRGM2 and IRGM3.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence
- Western blot (1 - 2 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 20 kDa Observed: 24 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRGM / LRG-47
Validation
Anti-IRGM antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IRGM antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ IRGM / LRG-47 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B2732 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Autophagy and mitochondrial remodelling in mouse mesenchymal stromal cells challenged with Staphylococcus epidermidis. McDaniel DP, Zhai M, Liao PJ, Garrison BR, Kiang JG. Journal of cellular and molecular medicine. Feb;
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Mouse
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
