Human HTT / Huntingtin
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot
- (applications tested for the base form of this product only)
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Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin.
Reacts with an epitope corresponding to the human HDC region (2703-2911 amino acids) of the huntingtin protein. Detects a 350kD band on Western Blots but also detects smaller degradation products of Huntingtin. Recognizes both denatured and native Huntingtin in human brain. The combined use of this antibody, LS-C24585 and LS-demonstrates that huntingtin is enriched in neuronal cells in the brain.
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Immunohistochemistry: Frozen, FFPE (human). Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol. Positive control: IHC frozen-rabbit btain.
PBS, 0.09% Sodium Azide
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.