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Catalog Number Size Price
LS-B16075-50 50 µl (1 mg/ml) $460 
HSPD1 / HSP60 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Immunofluorescent analysis of HSP60 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPD1 / HSP60 Antibody - Western blot analysis of HSP60 expression in HeLa (A); NIH3T3 (B); KNRK (C); L929 (D) whole cell lysates.
HSPD1 / HSP60 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Immunofluorescent analysis of HSP60 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPD1 / HSP60 Antibody - Western blot analysis of HSP60 expression in HeLa (A); NIH3T3 (B); KNRK (C); L929 (D) whole cell lysates.
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PathPlus™ Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (C‑Terminus, IHC, IF, WB) LS‑B16075

PathPlus™ Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (C‑Terminus, IHC, IF, WB) LS‑B16075

Note: This antibody replaces LS-C352351
Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16075-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HSPD1 heat shock protein family D member 1 (HSP60) is a member of the mitochondrial chaperonin family that is required for the folding and assembly of proteins in the mitochondria. HSPD1 is also an interferon regulatory factor 3-interacting protein whose overexpression enhances activation of the IFN-beta promoter, contributing to the induction of the IFN-beta signaling pathway. Mutations in this gene cause some forms of hereditary spastic paraplegia. It is expressed in the adrenal, kidney, skeletal muscle, and many other tissues. References: Front Mol biosci 2016 3:49; PlosOne 2014 9(12):e114874; Science Direct 2002 70(5):1328
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human HSP60.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of HSP60 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

Validation

HSPD1 / HSP60 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPD1 / HSP60 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPD1 / HSP60 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ HSPD1 / HSP60 Antibody (C-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B16075 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024