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Catalog Number Size Price
LS-B54-50 50 µg (1 mg/ml) $485 
HMGN1 / HMG14 Antibody - Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
HMGN1 / HMG14 Antibody - Anti-HMNG Antibody - Western Blot. Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time. Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.
HMGN1 / HMG14 Antibody - Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
HMGN1 / HMG14 Antibody - Anti-HMNG Antibody - Western Blot. Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time. Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.
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IHC‑plus™ Polyclonal Rabbit anti‑Human HMGN1 / HMG14 Antibody (phospho‑Ser20/24, IHC, WB) LS‑B54

IHC‑plus™ Polyclonal Rabbit anti‑Human HMGN1 / HMG14 Antibody (phospho‑Ser20/24, IHC, WB) LS‑B54

Note: This antibody replaces LS-C18917
Antibody:
HMGN1 / HMG14 Rabbit anti-Human Polyclonal (pSer20/24) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B54-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HMGN1 / HMG14 Rabbit anti-Human Polyclonal (pSer20/24) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HMG14 antibody LS-B54 is an unconjugated rabbit polyclonal antibody to human HMG14 (HMGN1) (pSer20/24). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HMGN1 / HMG14
Synonyms
HMGN1 | HMG14
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Mouse, Rat, Bovine, Dog, Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-28 of human HMGN protein (see below).
Epitope
pSer20/24
Specificity
Reactivity occurs against human HMGN pS20/pS24 proteins and the antibody is specific for the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with HMGN proteins from Xenopus, chicken, mouse, bovine, dog, orangutan, rat and swine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B54 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B54 was determined to be 20 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HMGN1 / HMG14
P05114 NM_004965 NP_004956.5

Validation

HMGN1 / HMG14 Antibody - Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

LSBio Ratings

IHC-plus™ HMGN1 / HMG14 Antibody (phospho-Ser20/24) for IHC, WB/Western, ELISA LS-B54 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022