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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3729-0.05 0.05 mg $425 
HLA-E Antibody - Anti-HLA-E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ Monoclonal HLA‑E Antibody (clone MEM‑E/06, IHC) LS‑B3729

IHCPlus™ Monoclonal HLA‑E Antibody (clone MEM‑E/06, IHC) LS‑B3729

Note: This antibody replaces LS-C46059
Antibody:
Mouse Monoclonal to Human HLA-E
Application:
IHC, IHC-P, IP, Flo
Reactivity:
Human, Non-Human Primates
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3729-0.05
0.05 mg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human HLA-E
Application:
IHC, IHC-P, IP, Flo
Reactivity:
Human, Non-Human Primates
Format:
Unconjugated, Unmodified

Specifications

Description
HLA-E antibody LS-B3729 is an unconjugated mouse monoclonal antibody to HLA-E from human and non-human primates. Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Target
Human HLA-E
Synonyms
HLA-E | EA2.1 | HLAE | MHC HLA-E alpha-2.1 | MHC HLA-E alpha-1 | EA1.2 | HLA-6.2 | MHC class I antigen E | QA1
Host
Mouse
Reactivity
Human, Non-Human Primates (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
MEM-E/06
Conjugations
Unconjugated
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
Specificity
The antibody MEM-E/06 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HLA-E
P13747 NM_005516 NP_005507.3

Validation

HLA-E Antibody - Anti-HLA-E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HLA-E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HLA-E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ HLA-E Antibody (clone MEM-E/06) for IHC, IP, Flow LS-B3729 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/6/2020