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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B1805-50 50 µg (0.5 mg/ml) $425 
HIPK3 / FIST Antibody - Anti-HIPK3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
HIPK3 / FIST Antibody - Western blot of HIPK3 inumouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3)?rat testis using antibody at 1 ug/ml.
HIPK3 / FIST Antibody - Anti-HIPK3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
HIPK3 / FIST Antibody - Western blot of HIPK3 inumouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3)?rat testis using antibody at 1 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human HIPK3 / FIST Antibody (aa850‑900, IHC, WB) LS‑B1805

IHC‑plus™ Polyclonal Rabbit anti‑Human HIPK3 / FIST Antibody (aa850‑900, IHC, WB) LS‑B1805

Note: This antibody replaces LS-C47376
Antibody:
HIPK3 / FIST Rabbit anti-Human Polyclonal (aa850-900) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B1805-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HIPK3 / FIST Rabbit anti-Human Polyclonal (aa850-900) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
FIST antibody LS-B1805 is an unconjugated rabbit polyclonal antibody to FIST (HIPK3) (aa850-900) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HIPK3 / FIST
Synonyms
HIPK3 | ANPK | FIST | FIST3 | Homolog of protein kinase YAK1 | DYRK6 | PKY | YAK1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A portion of amino acids 850-900of human HIPK3.
Epitope
aa850-900
Specificity
A portion of amino acids 850-900 of human HIPK3.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1805 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1805 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIPK3 / FIST
Q9H422 NM_005734 NP_005725.3

Validation

HIPK3 / FIST Antibody - Anti-HIPK3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HIPK3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HIPK3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ HIPK3 / FIST Antibody (aa850-900) for IHC, WB/Western LS-B1805 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 9/22/2020