Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B109-0.1 0.1 ml (1 mg/ml) $460 
HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC‑plus™ Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (clone ESEE122, aa329‑530, IHC, IF, WB) LS‑B109

IHC‑plus™ Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (clone ESEE122, aa329‑530, IHC, IF, WB) LS‑B109

Note: This antibody replaces LS-C2555, LS-C150386
Antibody:
HIF1A / HIF1 Alpha Mouse anti-Human Monoclonal (aa329-530) (ESEE122) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-B109-0.1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HIF1A / HIF1 Alpha Mouse anti-Human Monoclonal (aa329-530) (ESEE122) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HIF1 Alpha antibody LS-B109 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) (aa329-530) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
ESEE122
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human HIF-1 alpha amino acids 329-530.
Epitope
aa329-530
Specificity
HIF-1 alpha.
Applications
  • IHC
  • IHC - Paraffin (1.25 µg/ml)
  • IHC - Frozen (1:100 - 1:5000)
  • ICC
  • Immunofluorescence (8 - 12 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml.
Presentation
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

Validation

HIF1A / HIF1 Alpha Antibody - Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B109 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/10/2024