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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5244-50 | 50 µg (1 mg/ml) | $485 |
IHC‑plus™ Monoclonal Rat Heparan Sulfate Proteoglycan Antibody (IHC, WB) LS‑B5244
IHC‑plus™ Monoclonal Rat Heparan Sulfate Proteoglycan Antibody (IHC, WB) LS‑B5244
Note: This antibody replaces LS-C67430
Antibody:
Heparan Sulfate Proteoglycan Rat Monoclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Heparan Sulfate Proteoglycan Rat Monoclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Specifications
Description
Heparan Sulfate Proteoglycan antibody LS-B5244 is an unconjugated rat monoclonal antibody to Heparan Sulfate Proteoglycan from human, mouse, rat and other species. Validated for ICC, IHC, IP and WB.
Host
Rat
Reactivity
Human, Mouse, Rat, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and Heparan Sulfate Proteoglycan
Specificity
Reacts with domain IV of the core protein of the large heparan sulfate proteoglycan or perlecan. Reactivity is independent of the galactosaminoglycan moieties. Recognizes human Heparan Sulfate Proteoglycan, Large Species cross-reactivity: mouse, rat, and bovine.
Applications
- IHC
- IHC - Paraffin (10 - 20 µg/ml)
- IHC - Frozen (1:25 - 1:200)
- ICC
- Western blot (1:100 - 1:1000)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:100-1:1000. Immunohistochemistry (frozen and paraffin sections): 1:25-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as a detecting reagent. Optimum dilutions to be determined by researcher. Hybridoma: X63Ag8.653 mouse myeloma cells with spleen cells from a Fisher rat.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Aliquots are stable for 1 year after receipt.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-Heparan Sulfate Proteoglycan antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Heparan Sulfate Proteoglycan antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ Heparan Sulfate Proteoglycan Antibody for IHC, ICC, WB/Western, IP LS-B5244 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse, Mammal
Applications:
Western blot, ELISA, Radioimmunoassay
Species:
Mammal
Applications:
Western blot, ELISA, Radioimmunoassay
Reactivity:
Rat
Range:
31.2-2000 pg/ml
Reactivity:
Porcine
Range:
0.31-20 ng/ml
Reactivity:
Human
Range:
0.313-20 ng/ml
Reactivity:
Mouse
Range:
0.39-25 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
