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Catalog Number Size Price
LS-B55-50 50 µg (1 mg/ml) $485 
GSK3B / GSK3 Beta Antibody - Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GSK3B / GSK3 Beta Antibody - Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG (LS-C60865) for detection.
GSK3B / GSK3 Beta Antibody - Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GSK3B / GSK3 Beta Antibody - Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG (LS-C60865) for detection.
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IHC‑plus™ Polyclonal Rabbit anti‑Human GSK3B / GSK3 Beta Antibody (phospho‑Ser9, IHC, IF, WB) LS‑B55

IHC‑plus™ Polyclonal Rabbit anti‑Human GSK3B / GSK3 Beta Antibody (phospho‑Ser9, IHC, IF, WB) LS‑B55

Note: This antibody replaces LS-C18919, LS-C66828
Antibody:
GSK3B / GSK3 Beta Rabbit anti-Human Polyclonal (pSer9) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B55-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GSK3B / GSK3 Beta Rabbit anti-Human Polyclonal (pSer9) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GSK3 Beta antibody LS-B55 is an unconjugated rabbit polyclonal antibody to human GSK3 Beta (GSK3B) (pSer9). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human GSK3B / GSK3 Beta
Synonyms
GSK3B | GSK-3 beta | GSK3 beta | GSK3beta | GSK3beta isoform | TPKI | GSK 3beta | Tau protein kinase I | ZGSK-3beta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat, Dog, Chicken, Frog, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-15 of human GSK3 beta.
Epitope
pSer9
Specificity
This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B55 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B55 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GSK3B / GSK3 Beta
P49841 NM_002093 NP_002084.2

Validation

GSK3B / GSK3 Beta Antibody - Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ GSK3B / GSK3 Beta Antibody (phospho-Ser9) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B55 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Allodi I, Comley L, Nichterwitz S, Nizzardo M, Simone C, Benitez JA, Cao M, Corti S, Hedlund E. Scientific reports. 2016 6:25960. (WB; Human)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 8/11/2022