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IHCPlus™ GRK5 Antibody Internal Polyclonal IHC LS‑A3030

Catalog Number Size Price
LS-A3030-50 50 µg (0.35 mg/ml) $425 
Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ GRK5 Antibody Internal Polyclonal IHC LS‑A3030

Note: This antibody replaces LS-C67124
Antibody:
Rabbit Polyclonal to Human GRK5
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A3030-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GRK5
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GRK5 antibody LS-A3030 is an unconjugated rabbit polyclonal antibody to GRK5 from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human GRK5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken (tested or 100% immunogen sequence identity)
Predicted
Dog, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (7 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 15 amino acid peptide from internal region of human GRK5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Bovine, Panda, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%); Orangutan, Dog, Elephant, Xenopus (93%).
Blocking Peptide
LS-E28672 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A3030. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human GRK5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A3030 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3030 was determined to be 7 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GRK5
P34947 NM_005308 NP_005299.1

Validation

Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GRK5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 9/22/2019