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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A102-50 50 µg (1 mg/ml) $425 
GPR20 Antibody - Anti-GPR20 antibody IHC of human basal nucleus of Meynert. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ GPR20 Antibody N‑Terminus Polyclonal IHC LS‑A102

IHCPlus™ GPR20 Antibody N‑Terminus Polyclonal IHC LS‑A102

Antibody:
Rabbit Polyclonal to Human GPR20
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A102-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPR20
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR20 antibody LS-A102 is an unconjugated rabbit polyclonal antibody to human GPR20. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human GPR20
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from N-terminal extracellular domain of human GPR20. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Monkey (95%); Marmoset (80%).
Blocking Peptide
LS-E28098 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A102. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human GPR20. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR20
Q99678 NM_005293 NP_005284.2

Validation

GPR20 Antibody - Anti-GPR20 antibody IHC of human basal nucleus of Meynert. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR20 antibody IHC of human basal nucleus of Meynert. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR20 antibody IHC of human basal nucleus of Meynert. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 11/12/2019