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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IHCPlus™ GPR174 Antibody Cytoplasmic Domain Polyclonal ELISA,IHC LS‑A408

Catalog Number Size Price
LS-A408-50 50 µg (1 mg/ml) $425 
Anti-GPR174 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ GPR174 Antibody Cytoplasmic Domain Polyclonal ELISA,IHC LS‑A408

Antibody:
Rabbit Polyclonal to Human GPR174
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A408-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPR174
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR174 antibody LS-A408 is an unconjugated rabbit polyclonal antibody to GPR174 from human and monkey. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human GPR174
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human GPR174. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Bat, Bovine, Pig (89%); Marmoset, Dog, Panda, Horse, Rabbit (83%).
Epitope
Cytoplasmic Domain
Specificity
Human GPR174. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A408 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A408 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR174
Q9BXC1 NM_032553 NP_115942.1

Validation

Anti-GPR174 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPR174 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR174 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 8/19/2019
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