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IHCPlus™ GPER1 / GPR30 Antibody Extracellular Domain Polyclonal IF,IHC,WB LS‑A4271

Catalog Number Size Price
LS-A4271-50 50 µg (1 mg/ml) $425 
Anti-GPR30 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ GPER1 / GPR30 Antibody Extracellular Domain Polyclonal IF,IHC,WB LS‑A4271

Note: This antibody replaces LS-C66914
Antibody:
Rabbit Polyclonal to Human GPER1 / GPR30
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A4271-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPER1 / GPR30
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR30 antibody LS-A4271 is an unconjugated rabbit polyclonal antibody to GPR30 (GPER1 ) from human and monkey. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 8 publications.
Target
Human GPER1 / GPR30
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (6.4 µg/ml)
  • Immunofluorescence
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Dog, Opossum (94%); Panda, Bat, Horse, Rabbit, Platypus (88%).
Blocking Peptide
LS-E28932 - Liquid - 50 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-A4271. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Extracellular Domain
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Antibody does not work for Western blot with mouse samples.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPER1 / GPR30
Q99527 NM_001505 NP_001496.1

Validation

Anti-GPR30 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPR30 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR30 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Publications (8)

Identification of the estrogen receptor GPER in neoplastic and non-neoplastic human testes. Rago V, Romeo F, Giordano F, Maggiolini M, Carpino A. Reproductive biology and endocrinology : RB&E. 2011 9:135. (WB, IHC-P; Human)

Nuclear alternate estrogen receptor GPR30 mediates 17beta-estradiol-induced gene expression and migration in breast cancer-associated fibroblasts. Madeo A, Maggiolini M. Cancer research. 2010 Jul;70:6036-46.

Identification of G protein-coupled estrogen receptor in human and pig spermatozoa. Rago V, Giordano F, Brunelli E, Zito D, Aquila S, Carpino A. Journal of anatomy. 2014 Jun;224:732-6.

Expression pattern of G protein-coupled receptor 30 in LacZ reporter mice. Isensee J, Meoli L, Zazzu V, Nabzdyk C, Witt H, Soewarto D, Effertz K, Fuchs H, Gailus-Durner V, Busch D, Adler T, de Angelis MH, Irgang M, Otto C, Noppinger PR. Endocrinology. 2009 Apr;150:1722-30.

G protein-coupled receptor 30 expression is up-regulated by EGF and TGF alpha in estrogen receptor alpha-positive cancer cells. Vivacqua A, Lappano R, De Marco P, Sisci D, Aquila S, De Amicis F, Fuqua SA, And[Character f2] S, Maggiolini M. Molecular endocrinology (Baltimore, Md.). 2009 Nov;23:1815-26.

c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells. Madeo A, Vinciguerra M, Lappano R, Galgani M, Gasperi-Campani A, Maggiolini M, Musti AM. Oncogene. 2010 Feb;29:978-91.

Stallion spermatozoa: putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER. Arkoun B, Gautier C, Delalande C, Barrier-Battut I, Gu[Character e9]non I, Goux D, Boura[Character ef]ma-Lelong H. General and comparative endocrinology. 2014 May;200:35-43.

Estriol acts as a GPR30 antagonist in estrogen receptor-negative breast cancer cells. Lappano R, Rosano C, De Marco P, De Francesco EM, Pezzi V, Maggiolini M. Molecular and cellular endocrinology. 2010 May;320:162-70.

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Requested From: United States
Date Requested: 8/17/2019
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