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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ GPER1 / GPR30 Antibody C‑Terminus Polyclonal IF,IHC,WB LS‑A4272

Catalog Number Size Price
LS-A4272-50 50 µg (1 mg/ml) $425 
Anti-GPER1 / GPR30 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPER1 / GPR30 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-GPER1 / GPR30 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPER1 / GPR30 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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IHCPlus™ GPER1 / GPR30 Antibody C‑Terminus Polyclonal IF,IHC,WB LS‑A4272

Antibody:
Rabbit Polyclonal to Human GPER1 / GPR30
Application:
IHC, IHC-P, IHC-Fr, IF, WB
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A4272-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPER1 / GPR30
Application:
IHC, IHC-P, IHC-Fr, IF, WB
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR30 antibody LS-A4272 is an unconjugated rabbit polyclonal antibody to GPR30 (GPER1 ) from human, rat, dog and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 9 publications.
Target
Human GPER1 / GPR30
Host
Rabbit
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Predicted
Rat, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 18 amino acid peptide from C-terminus of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%); Rat, Horse (94%); Mouse, Hamster (89%); Elephant, Bovine (83%).
Blocking Peptide
LS-E28933 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A4272. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPER1 / GPR30
Q99527 NM_001505 NP_001496.1

Validation

Anti-GPER1 / GPR30 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPER1 / GPR30 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPER1 / GPR30 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-GPER1 / GPR30 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPER1 / GPR30 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPER1 / GPR30 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Publications (9)

Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming. Sahlin L, Stjernholm-Vladic Y, Roos N, Masironi B, Ekman-Ordeberg G. Reproductive biology and endocrinology : RB&E. 2008 6:36. (IHC-P; Human)

Spinal synthesis of estrogen and concomitant signaling by membrane estrogen receptors regulate spinal - and -opioid receptor heterodimerization and female-specific spinal morphine antinociception. Liu NJ, Chakrabarti S, Schnell S, Wessendorf M, Gintzler AR. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:11836-45. (IHC-Fr; Rat)

Regulation of ERRalpha gene expression by estrogen receptor agonists and antagonists in SKBR3 breast cancer cells: differential molecular mechanisms mediated by g protein-coupled receptor GPR30/GPER-1. Li Y, Birnbaumer L, Teng CT. Molecular endocrinology (Baltimore, Md.). 2010 May;24:969-80.

Stallion spermatozoa: putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER. Arkoun B, Gautier C, Delalande C, Barrier-Battut I, Gu[Character e9]non I, Goux D, Boura[Character ef]ma-Lelong H. General and comparative endocrinology. 2014 May;200:35-43.

Estradiol and GPER Activation Differentially Affect Cell Proliferation but Not GPER Expression in the Hippocampus of Adult Female Rats. Duarte-Guterman P, Lieblich SE, Chow C, Galea LA. PloS one. 2015 10:e0129880. (IF; Rat)

GPR30 regulates the EGFR-Akt cascade and predicts lower survival in patients with ovarian cancer. Fujiwara S, Terai Y, Kawaguchi H, Takai M, Yoo S, Tanaka Y, Tanaka T, Tsunetoh S, Sasaki H, Kanemura M, Tanabe A, Yamashita Y, Ohmichi M. Journal of ovarian research. 2012 5:35. (WB; Human)

G protein-coupled receptor 30 localizes to the endoplasmic reticulum and is not activated by estradiol. Otto C, Rohde-Schulz B, Schwarz G, Fuchs I, Klewer M, Brittain D, Langer G, Bader B, Prelle K, Nubbemeyer R, Fritzemeier KH. Endocrinology. 2008 149:4846-56. (IF; Human, Monkey, Hamster)

Severe pulmonary hypertension in aging female apolipoprotein E-deficient mice is rescued by estrogen replacement therapy. Umar S, Partow-Navid R, Ruffenach G, Iorga A, Moazeni S, Eghbali M. Biology of sex differences. 2017 March;8:9. (Human)

A genetic polymorphism repurposes the G-protein coupled and membrane-associated estrogen receptor GPER to a transcription factor-like molecule promoting paracrine signaling between stroma and breast carcinoma cells. Pupo M, Bodmer A, Berto M, Maggiolini M, Dietrich PY, Picard D. Oncotarget. 2017 July;8:46728-46744. (Human)

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Range: 0.312-20 ng/ml

Requested From: United States
Date Requested: 8/20/2019
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