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Catalog Number Size Price
LS-B15965-50 50 µg $460 
GNL3 / NS / Nucleostemin Antibody - Human SmallIntestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
GNL3 / NS / Nucleostemin Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
GNL3 / NS / Nucleostemin Antibody - Goat anti-GNL3 (aa115-126) Antibody (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
GNL3 / NS / Nucleostemin Antibody - Antibody (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GNL3 / NS / Nucleostemin Antibody - Human SmallIntestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
GNL3 / NS / Nucleostemin Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
GNL3 / NS / Nucleostemin Antibody - Goat anti-GNL3 (aa115-126) Antibody (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
GNL3 / NS / Nucleostemin Antibody - Antibody (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human GNL3 / NS / Nucleostemin Antibody (aa115‑126, IHC, WB) LS‑B15965

IHC‑plus™ Polyclonal Goat anti‑Human GNL3 / NS / Nucleostemin Antibody (aa115‑126, IHC, WB) LS‑B15965

Note: This antibody replaces LS-C742431
Antibody:
GNL3 / NS / Nucleostemin Goat anti-Human Polyclonal (aa115-126) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B15965-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GNL3 / NS / Nucleostemin Goat anti-Human Polyclonal (aa115-126) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nucleostemin antibody LS-B15965 is an unconjugated goat polyclonal antibody to human Nucleostemin (GNL3 / NS) (aa115-126). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GNL3 / NS / Nucleostemin
Synonyms
GNL3 | C77032 | E2-induced gene 3 protein | E2IG3 | NS | NNP47 | Nucleostemin | Novel nucleolar protein 47
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-TENKAKSGKQNS, from the internal region of the protein sequence according to NP_055181.3NP_996561.1.
Epitope
aa115-126
Specificity
This antibody is expected to recognize both reported isoforms (NP_055181.3; NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.
Applications
  • IHC - Paraffin
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 65kDa band observed in nuclear lysates of cell line K562 (calculated MW of 62.0kDa according to NP_055181.3). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GNL3 / NS / Nucleostemin
Q9BVP2 NM_014366 NP_055181.3

Validation

GNL3 / NS / Nucleostemin Antibody - Human SmallIntestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human SmallIntestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human SmallIntestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

GNL3 / NS / Nucleostemin Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ GNL3 / NS / Nucleostemin Antibody (aa115-126) for IHC, WB/Western LS-B15965 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024