Mouse GNAO1

Rabbit

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Ammonium sulfate precipitation

Unmodified

Synthetic peptide from mouse GNAO1 / GNAO.

aa345-354

Recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kD forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Has been used successfully for the detection of G(o) in Drosophila by western blotting.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot
• ELISA (1:500 - 1:1000)

Immunohistochemistry: LS-B3655 was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3655 was determined to be 10 ug/ml. Western Blot: This antibody detects band at 39 kD when used under reducing conditions against rat brain.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09471 NCBI: NM_020988 NP_066268.1