Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Reasearch Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

IHCPlus™ GDF15 Antibody Monoclonal ELISA,IHC,WB LS‑B8327

Catalog Number Size Price
LS-B8327-50 50 µg (1 mg/ml) $425 
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

IHCPlus™ GDF15 Antibody Monoclonal ELISA,IHC,WB LS‑B8327

Note: This antibody replaces LS-C153803
Antibody:
Rat Monoclonal to Mouse GDF15
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8327-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse GDF15
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
GDF15 antibody LS-B8327 is an unconjugated rat monoclonal antibody to GDF15 from human and mouse. Validated for ELISA, IHC and WB.
Target
Mouse GDF15
Host
Rat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
6D12.H10.E4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E. coli corresponding to full-length mouse NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
Specificity
This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Antibody reacts with human. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Usage
This Protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Expiration date is one year from opening.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GDF15
Q99988 NM_004864 NP_004855.2

Validation

Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
IHC Validated
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

Publications (0)

Reviews (0)

Featured Products

Product
Species: Human
Applications: Flow Cytometry, ELISA
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, Peptide Enzyme-Linked Immunosorbent Assay
Anti-GDF15 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-NAG-1 (D variant specific) Antibody - ELISA. In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 ug/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 ul per well at 1 ug/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.
Species: Human
Applications: IHC, Western blot, ELISA
Product
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Reactivity: Human
Range: 15.6-1000 pg/ml

Requested From: United States
Date Requested: 9/22/2019