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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B5722-100 100 µl $485 
FZD5 / Frizzled 5 Antibody - Anti-FZD5 / Frizzled 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FZD5 / Frizzled 5 Antibody - FZD5 / Frizzled 5 antibody Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FZD5 / Frizzled 5 Antibody - Anti-FZD5 / Frizzled 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FZD5 / Frizzled 5 Antibody - FZD5 / Frizzled 5 antibody Western blot of Fetal Stomach lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ Polyclonal Rabbit anti‑Human FZD5 / Frizzled 5 Antibody (aa467‑516, IHC, WB) LS‑B5722

IHCPlus™ Polyclonal Rabbit anti‑Human FZD5 / Frizzled 5 Antibody (aa467‑516, IHC, WB) LS‑B5722

Note: This antibody replaces LS-C110620
Antibody:
Rabbit Polyclonal to Human FZD5 / Frizzled 5
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5722-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human FZD5 / Frizzled 5
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Frizzled 5 antibody LS-B5722 is an unconjugated rabbit polyclonal antibody to Frizzled 5 (FZD5) (aa467-516) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FZD5 / Frizzled 5
Synonyms
FZD5 | C2orf31 | DKFZP434E2135 | Frizzled family receptor 5 | Fz5 | Fzd-5 | FzE5 | Frizzled 5 | Frizzled-5 | Wnt receptor | Fz-5 | HFZ5
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa467-516 of human FZD5 (Q13467, NP_003459). Percent identity by BLAST analysis: Human (100%); Gorilla (92%); Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Pig, Guinea pig (85%).
Blocking Peptide
LS-E15164 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5722. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa467-516
Specificity
Human FZD5 / Frizzled 5
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FZD5 / Frizzled 5
Q13467 NM_003468 NP_003459.2

Validation

FZD5 / Frizzled 5 Antibody - Anti-FZD5 / Frizzled 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FZD5 / Frizzled 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FZD5 / Frizzled 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ FZD5 / Frizzled 5 Antibody (aa467-516) for IHC, WB/Western LS-B5722 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 6/3/2020