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IHCPlus™ FMR1 / FMRP Antibody aa594‑607 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3953

Catalog Number Size Price
LS-B3953-50 50 µg (0.5 mg/ml) $425 
Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
FMR1 / FMRP antibody (0.1µg/ml) staining of KELLY cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
FMR1 / FMRP antibody (0.1µg/ml) staining of KELLY cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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IHCPlus™ FMR1 / FMRP Antibody aa594‑607 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3953

Note: This antibody replaces LS-C87208
Antibody:
Goat Polyclonal to Human FMR1 / FMRP
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Hamster, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3953-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human FMR1 / FMRP
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Hamster, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
FMRP antibody LS-B3953 is an unconjugated goat polyclonal antibody to FMRP (FMR1 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FMR1 / FMRP
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-RTGKDRNQKKEKPD, from the internal region (near the C Terminus) of the protein sequence according to NP_002015.1.
Blocking Peptide
LS-E26378 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3953. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa594-607
Specificity
Human FMR1.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 80kD band observed in lysates of cell line KELLY (calculated MW of 71.2kD according to NP_002015.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Genet. 1996 Jan;5(1):95-102; PMID: 8789445). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FMR1 / FMRP
Q06787 NM_002024 NP_002015.1

Validation

Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
IHC Validated
Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

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Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded human brain tissues, showing cytoplasmic localization with DAB staining using FMR1 mouse monoclonal antibody.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Immunoprecipitation - Drosophila FMR1 antibody [6A15]  using tissue lysate from Drosophila cellularizing embryos
Species: Drosophila
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Reactivity: Human
Range: 0.313-20 ng/ml

Requested From: United States
Date Requested: 9/22/2019