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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B6053-50 50 µl $485 
FLT4 / VEGFR3 Antibody - Anti-VEGFR3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHC‑plus™ Monoclonal Mouse anti‑Human FLT4 / VEGFR3 Antibody (clone 9D9F9, Extracellular Domain, IHC, WB) LS‑B6053

IHC‑plus™ Monoclonal Mouse anti‑Human FLT4 / VEGFR3 Antibody (clone 9D9F9, Extracellular Domain, IHC, WB) LS‑B6053

Note: This antibody replaces LS-C40133
Antibody:
FLT4 / VEGFR3 Mouse anti-Human Monoclonal (Extracellular Domain) (9D9F9) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B6053-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
FLT4 / VEGFR3 Mouse anti-Human Monoclonal (Extracellular Domain) (9D9F9) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
VEGFR3 antibody LS-B6053 is an unconjugated mouse monoclonal antibody to human VEGFR3 (FLT4) (Extracellular Domain). Validated for ELISA, Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FLT4 / VEGFR3
Synonyms
FLT4 | FLT41 | Fms-related tyrosine kinase 4 | Fms-like tyrosine kinase 4 | LMPH1A | PCL | Soluble VEGFR3 variant 1 | Vegf-r3 | VEGFR3 | Soluble VEGFR3 variant 2 | Soluble VEGFR3 variant 3 | FLT-4 | Quek2 | VEGF Receptor 3 | VEGFR-3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
9D9F9
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant human VEGFR-3 extracellular domain protein expressed in baculovirus culture.
Epitope
Extracellular Domain
Specificity
Recognizes human VEGF Receptor 3 (FLT4). Western blot and ELISA demonstrate that the antibody is highly specific for VEGFR-3 with no cross-reactivity with human VEGFR-1 or VEGFR-2 protein. VEGFR-3 is not expressed in HEVs of the lymph nodes. In lymphomas staining is seen mostly in collapsed vessels in the cortex of lymph nodes infiltrated by the lymphoma cells. The antibody specifically detects lymphatic endothelium in human lymph node and other organs such as tonsil.
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • IHC - Frozen (1:100 - 1:1000)
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Flow Cytometry, Immunoprecipitation, ELISA, Western Blot and Immunohistochemistry. Immunohistochemistry (Frozen): 1:100-1:1000. Cold acetone fixation. Immunoprecipitation: 1-5 ul per 300-500 ul of cell lysate. Use protein A or rabbit anti-mouse IgG. Optimal dilutions will need to be determined by the end-user. Positive control: Human lymphatic tissues.
Presentation
Supplied as a liquid, preservative free.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FLT4 / VEGFR3
P35916 NM_002020 NP_002011.2

Validation

FLT4 / VEGFR3 Antibody - Anti-VEGFR3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-VEGFR3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-VEGFR3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ FLT4 / VEGFR3 Antibody (Extracellular Domain, clone 9D9F9) for IHC, WB/Western, IP, Flow, ELISA LS-B6053 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/1/2020