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IHCPlus™ FER Antibody Monoclonal ELISA,IF,IHC,WB LS‑B3467

Catalog Number Size Price
LS-B3467-50 50 µl $425 
Anti-FER antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FER antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Confocal immunofluorescence of HeLa cells using FER mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Western blot using FER mouse monoclonal antibody against NIH/3T3 (1), A549 (2) and SK-MEL-5 (3) cell lysate.
Anti-FER antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FER antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Confocal immunofluorescence of HeLa cells using FER mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Western blot using FER mouse monoclonal antibody against NIH/3T3 (1), A549 (2) and SK-MEL-5 (3) cell lysate.
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IHCPlus™ FER Antibody Monoclonal ELISA,IF,IHC,WB LS‑B3467

Note: This antibody replaces LS-C41507, LS-C171381
Antibody:
Mouse Monoclonal to Human FER
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3467-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human FER
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FER antibody LS-B3467 is an unconjugated mouse monoclonal antibody to human FER. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human FER
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
5D2C4
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human FER expressed in E. coli.
Specificity
Human, Mouse
Usage
Immunohistochemistry: LS-B3467 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3467 was determined to be 1:200.
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FER
P16591 NM_005246 NP_005237.2

Validation

Anti-FER antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-FER antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FER antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Anti-FER antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-FER antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FER antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Publications (1)

FER overexpression is associated with poor postoperative prognosis and cancer-cell survival in non-small cell lung cancer. Kawakami M, Morita S, Sunohara M, Amano Y, Ishikawa R, Watanabe K, Hamano E, Ohishi N, Nakajima J, Yatomi Y, Nagase T, Fukayama M, Takai D. International journal of clinical and experimental pathology. 2013 6:598-612.

Reviews (0)

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Western blot of Fer antibody
Species: Human, Mouse, Rat
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Range: 0.313-20 ng/ml
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Reactivity: Rabbit
Range: 15.625-1000 pg/ml
Reactivity: Mouse
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Requested From: United States
Date Requested: 9/22/2019