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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A103455-100 100 µl $650 
FAK / Focal Adhesion Kinase Antibody - Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FAK / Focal Adhesion Kinase Antibody - Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FAK / Focal Adhesion Kinase Antibody - Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FAK / Focal Adhesion Kinase Antibody - Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FAK / Focal Adhesion Kinase Antibody - Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
FAK / Focal Adhesion Kinase Antibody - Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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IHCPlus™ FAK / Focal Adhesion Kinase Antibody C‑Terminus Polyclonal Azide‑free HRP IHC LS‑A103455

IHCPlus™ FAK / Focal Adhesion Kinase Antibody C‑Terminus Polyclonal Azide‑free HRP IHC LS‑A103455

Antibody:
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse
Format:
Horseradish Peroxidase, Azide-free
Other formats:
Price
Catalog Number
$650 
LS-A103455-100
100 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse
Format:
Horseradish Peroxidase, Azide-free
Other formats:

Specifications

Description
Focal Adhesion Kinase antibody LS-A103455 is an HRP-conjugated rabbit polyclonal antibody to Focal Adhesion Kinase (FAK ) from human, mouse and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human FAK / Focal Adhesion Kinase
Host
Rabbit
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with AP, APC, Biotin, FITC, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 16 amino acid peptide from C-terminus of human FAK. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse (100%); Rat, Dog, Bat, Bovine, Elephant, Horse, Rabbit, Opossum, Platypus (94%); Hamster, Xenopus (88%); Turkey, Chicken, Pufferfish (81%).
Blocking Peptide
LS-E28660 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A103455. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human FAK. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 6 ug/ml.
Presentation
PBS
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FAK / Focal Adhesion Kinase
Q05397 NM_005607 NP_005598.3

Validation

FAK / Focal Adhesion Kinase Antibody - Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

FAK / Focal Adhesion Kinase Antibody - Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

FAK / Focal Adhesion Kinase Antibody - Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 11/14/2019