Human EZH2

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.

Internal

Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.5 - 2 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2528 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2528 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Bands at ~100kD and ~125kD.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15910 NCBI: NM_004456 NP_004447.2