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IHCPlus™ EZH2 Antibody Internal Polyclonal ELISA,IHC,WB LS‑B2528

Catalog Number Size Price
LS-B2528-50 50 µg (0.25 mg/ml) $485 
Anti-EZH2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ EZH2 Antibody Internal Polyclonal ELISA,IHC,WB LS‑B2528

Note: This antibody replaces LS-C6747
Antibody:
Rabbit Polyclonal to Human EZH2
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B2528-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EZH2
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EZH2 antibody LS-B2528 is an unconjugated rabbit polyclonal antibody to human EZH2. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EZH2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.
Epitope
Internal
Specificity
Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.
Usage
Immunohistochemistry: LS-B2528 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2528 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Bands at ~100kD and ~125kD.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EZH2
Q15910 NM_004456 NP_004447.2

Validation

Anti-EZH2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-EZH2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EZH2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/23/2019