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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B3904-50 50 µl $425 
EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
EPHB2 / EPH Receptor B2 Antibody - Immunofluorescence of HeLa (left) and HepG2 (right) cells using EphB2 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
EPHB2 / EPH Receptor B2 Antibody - Western blot using EphB2 mouse monoclonal antibody against truncated EphB2 recombinant protein (1) and extracellular EphB2(aa19-476)-hIgGFc transfected CHO-K1 cell lysate(2).
EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
EPHB2 / EPH Receptor B2 Antibody - Immunofluorescence of HeLa (left) and HepG2 (right) cells using EphB2 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
EPHB2 / EPH Receptor B2 Antibody - Western blot using EphB2 mouse monoclonal antibody against truncated EphB2 recombinant protein (1) and extracellular EphB2(aa19-476)-hIgGFc transfected CHO-K1 cell lysate(2).
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IHCPlus™ Monoclonal Mouse anti‑Human EPHB2 / EPH Receptor B2 Antibody (clone 2D12C6, aa17‑200, IHC, IF, WB) LS‑B3904

IHCPlus™ Monoclonal Mouse anti‑Human EPHB2 / EPH Receptor B2 Antibody (clone 2D12C6, aa17‑200, IHC, IF, WB) LS‑B3904

Note: This antibody replaces LS-C108801
Antibody:
Mouse Monoclonal to Human EPHB2 / EPH Receptor B2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3904-50
50 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human EPHB2 / EPH Receptor B2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EPH Receptor B2 antibody LS-B3904 is an unconjugated mouse monoclonal antibody to human EPH Receptor B2 (EPHB2) (aa17-200). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EPHB2 / EPH Receptor B2
Synonyms
EPHB2 | CAPB | Cek5 | EK5 | ETECK | EPH tyrosine kinase 3 | DRT | ELK-related tyrosine kinase | Hek5 | EPH-like kinase 5 | EPHT3 | Nuk | PCBC | Tyrosine-protein kinase TYRO5 | Protein-tyrosine kinase HEK5 | Tyro5 | EPH receptor B2 | Ephrin type-B receptor 2 | EPTH3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
2D12C6
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:400)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.
Epitope
aa17-200
Specificity
Human
Usage
Immunohistochemistry: LS-B3904 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3904was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPHB2 / EPH Receptor B2
P29323 NM_017449 AAB94602.1

Validation

EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

EPHB2 / EPH Receptor B2 Antibody - Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-EPHB2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

LSBio Ratings

IHC-plus™ EPHB2 / EPH Receptor B2 Antibody (aa17-200, clone 2D12C6) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3904 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 8/10/2020