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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ EP300 / p300 Antibody aa1572‑2371 Monoclonal IF,IHC,IP,WB LS‑B8325

Catalog Number Size Price
LS-B8325-200 200 µl $425 
Anti-EP300 / p300 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
p300 detected in a HeLa nuclear extract using EP300 / p300 Antibody (1:1000). ECL: 20 minute exposure.
Anti-EP300 / p300 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
p300 detected in a HeLa nuclear extract using EP300 / p300 Antibody (1:1000). ECL: 20 minute exposure.
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IHCPlus™ EP300 / p300 Antibody aa1572‑2371 Monoclonal IF,IHC,IP,WB LS‑B8325

Note: This antibody replaces LS-C2746
Antibody:
Mouse Monoclonal to Human EP300 / p300
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Mustelid, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8325-200
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human EP300 / p300
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Mustelid, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
p300 antibody LS-B8325 is an unconjugated mouse monoclonal antibody to p300 (EP300 ) from human, mouse, rat and other species. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EP300 / p300
Host
Mouse
Reactivity
Human, Mouse, Rat, Mustelid, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
RW109
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein containing residues 1572-2371 of human p300.
Epitope
aa1572-2371
Specificity
P300 protein. This recognizes residues 2107-2283.
Presentation
0.1% Sodium Azide
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EP300 / p300
Q09472 NM_001429 NP_001420.2

Validation

Anti-EP300 / p300 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC Validated
Anti-EP300 / p300 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-EP300 / p300 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

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Anti-EP300 / p300 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Mustelid, Primate
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Anti-EP300 / p300 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
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Species: Human, Mouse, Rat
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Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ChIP-sequencing
Reactivity: Human, Mouse, Rat
Range: Positive/Negative

Requested From: United States
Date Requested: 8/22/2019
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