Human EIF3S5 / EIF3F

Rabbit

Human, Chimpanzee, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)

Human, Chimpanzee, Mouse, Rat, Bovine, Chicken (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human eIF3f protein.

A BLAST analysis was used to suggest cross reactivity with eIF3f protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against eIF3f from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with eIF3f from these sources. Reactivity against homologues from other sources is not known.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:200 - 1:2000)
• ELISA (1:10000 - 1:40000)

Immunohistochemistry: LS-B366 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B366 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00303 NCBI: NM_003754 NP_003745.1