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IHCPlus™ EGLN1 / PHD2 Antibody aa1‑50 Polyclonal Flo,IHC,WB LS‑B4810

Catalog Number Size Price
LS-B4810-100 100 µl (1 mg/ml) $425 
Anti-EGLN1 / PHD2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EGLN1 / PHD2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10 ng/lane) and whole cell lysate from HeLa cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EGLN1 / PHD2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EGLN1 / PHD2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10 ng/lane) and whole cell lysate from HeLa cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ EGLN1 / PHD2 Antibody aa1‑50 Polyclonal Flo,IHC,WB LS‑B4810

Note: This antibody replaces LS-C48986
Antibody:
Rabbit Polyclonal to Human EGLN1 / PHD2
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B4810-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EGLN1 / PHD2
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PHD2 antibody LS-B4810 is an unconjugated rabbit polyclonal antibody to PHD2 (EGLN1 ) from human and primate. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EGLN1 / PHD2
Host
Rabbit
Reactivity
Human, Primate (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2500)
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The epitope recognized by this antibody maps to a region between residues 1 and 50 of human Prolyl Hydroxylase Domain-Containing Protein 2 using the numbering given in entry NP_071334.1 (GeneID 54583)
Epitope
aa1-50
Specificity
This antibody is specific for human HIF prolyl hydroxylase 2.
Presentation
Tris-citrate/phosphate buffer, pH 7 to 8, 0.09% sodium azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGLN1 / PHD2
Q9GZT9 NM_022051 NP_071334.1

Validation

Anti-EGLN1 / PHD2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-EGLN1 / PHD2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EGLN1 / PHD2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-EGLN1 / PHD2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-EGLN1 / PHD2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EGLN1 / PHD2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Anti-EGLN1 / PHD2 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin
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Species: Human
Applications: Western blot
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Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Immunohistochemical analysis of EGLN1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Reactivity: Mouse
Range: 0.313-20 ng/ml

Requested From: United States
Date Requested: 9/22/2019