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IHCPlus™ DPYSL2 / CRMP2 Antibody aa463‑492 Monoclonal IHC,WB LS‑B3616

Catalog Number Size Price
LS-B3616-200 200 µl $425 
Anti-CRMP-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRMP-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot of lysate from mouse NIH/3T3 cell line, using DRP-2 Antibody(33CT11. 7. 6). 33CT11. 7. 6 was diluted at 1:2000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
Anti-CRMP-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRMP-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot of lysate from mouse NIH/3T3 cell line, using DRP-2 Antibody(33CT11. 7. 6). 33CT11. 7. 6 was diluted at 1:2000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
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IHCPlus™ DPYSL2 / CRMP2 Antibody aa463‑492 Monoclonal IHC,WB LS‑B3616

Note: This antibody replaces LS-C96448
Antibody:
Mouse Monoclonal to Human DPYSL2 / CRMP2
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3616-200
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human DPYSL2 / CRMP2
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CRMP2 antibody LS-B3616 is an unconjugated mouse monoclonal antibody to CRMP2 (DPYSL2 ) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human DPYSL2 / CRMP2
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Chicken, Cow (at least 90% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
33CT11.40.3
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa463-492
Specificity
This DRP-2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 463-492 amino acids from human DRP-2.
Usage
Immunohistochemistry: LS-B3616 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3616 was determined to be 1:200. Please note that we do not recommend the use of mouse antibodies with mouse tissues in IHC due to the likelihood of high background.
Presentation
0.9% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DPYSL2 / CRMP2
Q16555 NM_001386 NP_001377.1

Validation

Anti-CRMP-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-CRMP-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRMP-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Anti-CRMP-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-CRMP-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CRMP-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Publications (1)

Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates. Stratmann B, Engelbrecht B, Espelage BC, Klusmeier N, Tiemann J, Gawlowski T, Mattern Y, Eisenacher M, Meyer HE, Rabbani N, Thornalley PJ, Tschoepe D, Poschmann G, Stühler K. Scientific reports. 2016 6:37737. (WB; Human)

Reviews (0)

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Requested From: United States
Date Requested: 9/22/2019