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IHCPlus™ DCTN1 / Dynactin 1 Antibody aa1266‑1278 Polyclonal ICC,IF,IHC,Peptide‑ELISA,WB LS‑B2275

Catalog Number Size Price
LS-B2275-50 50 µg (0.5 mg/ml) $425 
Anti-DCTN1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
DCTN1 / Dynactin 1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DCTN1 / Dynactin 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Dynactin Antibody (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
DCTN1 / Dynactin 1 antibody staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
DCTN1 / Dynactin 1 antibody staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
Anti-DCTN1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
DCTN1 / Dynactin 1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DCTN1 / Dynactin 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Dynactin Antibody (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
DCTN1 / Dynactin 1 antibody staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
DCTN1 / Dynactin 1 antibody staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
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IHCPlus™ DCTN1 / Dynactin 1 Antibody aa1266‑1278 Polyclonal ICC,IF,IHC,Peptide‑ELISA,WB LS‑B2275

Note: This antibody replaces LS-C54747, LS-C65071
Antibody:
Goat Polyclonal to Human DCTN1 / Dynactin 1
Application:
IHC, IHC-P, ICC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Hamster, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2275-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human DCTN1 / Dynactin 1
Application:
IHC, IHC-P, ICC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Hamster, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
Dynactin 1 antibody LS-B2275 is an unconjugated goat polyclonal antibody to Dynactin 1 (DCTN1 ) from human, mouse, hamster and other species. Validated for ICC, IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DCTN1 / Dynactin 1
Host
Goat
Reactivity
Human, Monkey, Mouse, Hamster, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Immunofluorescence (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2NP_075408.1NP_001128512.1NP_001128513.1NP_001177765.1NP_001177766.1.
Blocking Peptide
LS-E25430 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2275. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1266-1278
Specificity
Human DCTN1. This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Usage
Immunohistochemistry: LS-B2275 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2275 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DCTN1 / Dynactin 1
Q14203 NM_023019 NP_075408.1

Validation

Anti-DCTN1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-DCTN1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DCTN1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/18/2019