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Catalog Number Size Price
LS-B2556-50 50 µg (1 mg/ml) $545 
CR1 / CD35 Antibody - Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
CR1 / CD35 Antibody - Anti-CD35 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
CR1 / CD35 Antibody - Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
CR1 / CD35 Antibody - Anti-CD35 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
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IHC‑plus™ Monoclonal Mouse anti‑Human CR1 / CD35 Antibody (clone E11, Preservative Free, IHC) LS‑B2556

IHC‑plus™ Monoclonal Mouse anti‑Human CR1 / CD35 Antibody (clone E11, Preservative Free, IHC) LS‑B2556

Note: This antibody replaces LS-C45218
Antibody:
CR1 / CD35 Mouse anti-Human Monoclonal (Preservative Free) (E11) Antibody
Application:
IHC, IHC-P, IHC-Fr, Flo
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Other formats:
Price
Catalog Number
$545
LS-B2556-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CR1 / CD35 Mouse anti-Human Monoclonal (Preservative Free) (E11) Antibody
Application:
IHC, IHC-P, IHC-Fr, Flo
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Other formats:

Specifications

Description
CD35 antibody LS-B2556 is an unconjugated mouse monoclonal antibody to human CD35 (CR1). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CR1 / CD35
Synonyms
CR1 | C3b/C4b receptor | C3-binding protein | CD35 | C3BR | Knops blood group antigen | C4BR | CD35 antigen | Complement receptor type 1 | KN
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
E11
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Preservative Free. Also available Unmodified.
Immunogen
Human acute monocytic leukaemia cells.
Specificity
Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Flow Cytometry (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: LS-B2556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2556 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.
Presentation
PBS
Storage
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CR1 / CD35
P17927 NM_000651 NP_000642.3

Validation

CR1 / CD35 Antibody - Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

CR1 / CD35 Antibody - Anti-CD35 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ CR1 / CD35 Antibody (clone E11, Preservative Free) for IHC, Flow LS-B2556 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022