Mammal Collagen V

Rabbit

Mammal, Human, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Affinity chromatography

Unmodified

Collagen Type V from human and bovine placenta.

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

• IHC
• IHC - Paraffin (1.25 µg/ml)
• Western blot (1:5000 - 1:10000)
• Immunoprecipitation (1:100)
• ELISA (1:4000 - 1:8000)

Immunohistochemistry: LS-B379 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B379 was determined to be 1.25 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.