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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B8473-50 50 µg (1 mg/ml) $425 
COL5A1 / Collagen V Alpha 1 Antibody - Anti-Collagen V antibody IHC of human skin, dermal collagen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

IHCPlus™ Monoclonal Mouse anti‑Human COL5A1 / Collagen V Alpha 1 Antibody (clone 13F6, IHC, WB) LS‑B8473

IHCPlus™ Monoclonal Mouse anti‑Human COL5A1 / Collagen V Alpha 1 Antibody (clone 13F6, IHC, WB) LS‑B8473

Note: This antibody replaces LS-C154757
Antibody:
Mouse Monoclonal to Human COL5A1 / Collagen V Alpha 1
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8473-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human COL5A1 / Collagen V Alpha 1
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen V Alpha 1 antibody LS-B8473 is an unconjugated mouse monoclonal antibody to human Collagen V Alpha 1 (COL5A1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human COL5A1 / Collagen V Alpha 1
Synonyms
COL5A1 | Collagen alpha-1(V) chain | Collagen, type V, alpha 1 | Collagen alpha 1(v) | Collagen V | Alpha 1 type V collagen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
13F6
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:200 - 1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant N alpha 1 fragment : Human N alpha 1 (V)
Specificity
Reacts with human pro alpha 1(V) chain. The epitope is located in the COL2-NC2 region. Cross reactivity with other forms of collagen has not been tested.
Presentation
Lyophilized
Reconstitution
Reconstitute in sterile distilled water.
Storage
Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at 4°C. For extended periods store in aliquots at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL5A1 / Collagen V Alpha 1
P20908 NM_000093 BAA14323.1

Validation

COL5A1 / Collagen V Alpha 1 Antibody - Anti-Collagen V antibody IHC of human skin, dermal collagen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-Collagen V antibody IHC of human skin, dermal collagen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-Collagen V antibody IHC of human skin, dermal collagen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

LSBio Ratings

IHC-plus™ COL5A1 / Collagen V Alpha 1 Antibody (clone 13F6) for IHC, WB/Western, IP, ELISA LS-B8473 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 5/31/2020