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IHCPlus™ CNTN1 / gp135 / Contactin 1 Antibody aa313‑324 Polyclonal IHC,Peptide‑ELISA,WB LS‑B10585

Catalog Number Size Price
LS-B10585-50 50 µg (0.5 mg/ml) $425 
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CNTN1 / gp135 / Contactin 1 antibody (0.3µg/ml) staining of Pig Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CNTN1 / gp135 / Contactin 1 antibody (0.3µg/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CNTN1 / gp135 / Contactin 1 antibody (0.3µg/ml) staining of Pig Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CNTN1 / gp135 / Contactin 1 antibody (0.3µg/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
CNTN1 antibody (0.3 ug/ml) staining of Human Cerebellum (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ CNTN1 / gp135 / Contactin 1 Antibody aa313‑324 Polyclonal IHC,Peptide‑ELISA,WB LS‑B10585

Note: This antibody replaces LS-C204997
Antibody:
Goat Polyclonal to Human CNTN1 / gp135 / Contactin 1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Guinea pig, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10585-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CNTN1 / gp135 / Contactin 1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Guinea pig, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Contactin 1 antibody LS-B10585 is an unconjugated goat polyclonal antibody to Contactin 1 (CNTN1 / gp135 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CNTN1 / gp135 / Contactin 1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-ENIRGKDKHQAR, from the internal region of the protein sequence according to NP_001834.2NP_778203.1NP_001242992.1.
Blocking Peptide
LS-E46930 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10585. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa313-324
Specificity
Human CNTN1 / gp135 / Contactin 1. This antibody is expected to recognize all reported isoforms (NP_001834.2; NP_778203.1; NP_001242992.1). Reported variants represent identical protein: NP_001242993.1, NP_001242992.1.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Human, Mouse and Rat Brain lysates (calculated MW of 113kD according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CNTN1 / gp135 / Contactin 1
Q12860 NM_001843 NP_001834.2

Validation

Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Featured Products

CNTN1 / Contactin 1 antibody Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: Western blot
Anti-CNTN1 / gp135 / Contactin 1 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CNTN1 / Contactin 1 antibody Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: Western blot
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC - Paraffin, Western blot

Requested From: United States
Date Requested: 9/19/2019