Human CLDN7 / Claudin 7

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide derived from the C-Terminus of the human claudin-7 protein

C-Terminus

Specific for the claudin-7 protein and does not cross-react with Claudin-3 or -4. On Western blots, it identifies a band at ~22kD. Reactivity has been confirmed with T47D cells by Western blotting and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1 µg/ml)
• Immunoprecipitation
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2918 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2918 was determined to be 10 ug/ml. Positive control: Western Blot: T47D cells.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95471 NCBI: NM_001307 NP_001298.3