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IHCPlus™ CHRM3 / M3 Antibody Cytoplasmic Domain Polyclonal Biotin ELISA,IHC LS‑A101125

Catalog Number Size Price
LS-A101125-100 100 µl $650 
Anti-CHRM3 / M3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ CHRM3 / M3 Antibody Cytoplasmic Domain Polyclonal Biotin ELISA,IHC LS‑A101125

Antibody:
Rabbit Polyclonal to Human CHRM3 / M3
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A101125-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CHRM3 / M3
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
M3 antibody LS-A101125 is a biotin-conjugated rabbit polyclonal antibody to M3 (CHRM3 ) from human, mouse, rat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CHRM3 / M3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with AP, APC, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum (100%); Guinea pig, Turkey, Chicken, Platypus, Lizard (94%).
Epitope
Cytoplasmic Domain
Specificity
Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A1424 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1424 was determined to be 5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHRM3 / M3
P20309 NM_000740 NP_000731.1

Validation

Anti-CHRM3 / M3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-CHRM3 / M3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM3 / M3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Species: Human
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Requested From: United States
Date Requested: 9/19/2019