Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-A101106-100 100 µl $650 
CHRM3 / M3 Antibody - Anti-CHRM3 / M3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ CHRM3 / M3 Antibody Cytoplasmic Domain Polyclonal APC ELISA,IHC LS‑A101106

IHCPlus™ CHRM3 / M3 Antibody Cytoplasmic Domain Polyclonal APC ELISA,IHC LS‑A101106

Antibody:
Rabbit Polyclonal to Human CHRM3 / M3
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A101106-100
100 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CHRM3 / M3
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
M3 antibody LS-A101106 is an APC-conjugated rabbit polyclonal antibody to M3 (CHRM3 ) from human and monkey. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CHRM3 / M3
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Allophycocyanin. Also available Unconjugated or conjugated with AP, Biotin, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Orangutan, Sheep, Bovine, Hamster, Horse (94%); Elephant, Pig (89%); Mouse, Rat, Dog, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard (83%).
Blocking Peptide
LS-E28269 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A101106. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHRM3 / M3
P20309 NM_000740 NP_000731.1

Validation

CHRM3 / M3 Antibody - Anti-CHRM3 / M3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM3 / M3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CHRM3 / M3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/13/2019