IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:400. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Positive control: Tonsil, B cell lymphoma . Cellular localization: Membrane, cytoplasm.
Tissue culture supernatant in TBS, pH 7.5, 1% BSA, 0.1% sodium azide.
Store at 2°C to 8°C. Do not freeze.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.