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Catalog Number Size Price
LS-B336-50 50 µg (1 mg/ml) $460 
CCNL2 / Cyclin L2 Antibody - Anti-CCNL2 / Cyclin L2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CCNL2 / Cyclin L2 Antibody - Western blot of Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
CCNL2 / Cyclin L2 Antibody - Anti-CCNL2 / Cyclin L2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CCNL2 / Cyclin L2 Antibody - Western blot of Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CCNL2 / Cyclin L2 Antibody (aa309‑384, IHC, WB) LS‑B336

IHC‑plus™ Polyclonal Rabbit anti‑Human CCNL2 / Cyclin L2 Antibody (aa309‑384, IHC, WB) LS‑B336

Note: This antibody replaces LS-C19032
Antibody:
CCNL2 / Cyclin L2 Rabbit anti-Human Polyclonal (aa309-384) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B336-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CCNL2 / Cyclin L2 Rabbit anti-Human Polyclonal (aa309-384) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin L2 antibody LS-B336 is an unconjugated rabbit polyclonal antibody to Cyclin L2 (CCNL2) (aa309-384) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CCNL2 / Cyclin L2
Synonyms
CCNL2 | ANIA-6B | CCNM | Cyclin M | HLA-ISO | PCEE | SB138 | Cyclin L2 | Cyclin-L2 | HCLA-ISO
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Epitope
aa309-384
Specificity
Synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B336 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B336 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCNL2 / Cyclin L2
Q96S94 NM_030937 NP_112199.2

Validation

CCNL2 / Cyclin L2 Antibody - Anti-CCNL2 / Cyclin L2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CCNL2 / Cyclin L2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CCNL2 / Cyclin L2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CCNL2 / Cyclin L2 Antibody (aa309-384) for IHC, WB/Western, ELISA LS-B336 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Role of CDK/cyclin complexes in transcription and RNA splicing. Loyer P, Trembley JH, Katona R, Kidd VJ, Lahti JM. Cellular signalling. 2005 17:1033-51.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/16/2021